Role of Different Transporting Systems in the Secretion of Alkaloids by Hairy Roots of Catharanthus roseus (L) G. Don
DOI:
https://doi.org/10.29356/jmcs.v59i2.24Keywords:
ABC transporters, alkaloids, Catharanthus roseus, exudation, inhibitors.Abstract
Knowledge on the biosynthetic pathways of the monoterpene alkaloids is enormous, but little is known about their mechanism of transporting system from the plant cell. There is not concrete evidence confirming the role of ABC transporters in the secretion of monoterpene indole alkaloids (MIAs) in Catharanthus roseus. Therefore, in order to determine the role of different transporting systems involved in the MIAs translocation, we employed a pharmacological approach by using transport inhibitors such as, KCN, Na3VO4, quinidine and glibenclamide in hairy root cultures of C. roseus. It was found that the accumulation of ATP drastically decreased in the presence of KCN or 100 μM acetylsalicylic acid (ASA)/100 μM KCN. The treatment with the inhibitors KCN and glibenclamide in the presence of ASA significantly increased the ajmalicine secretion compared to the control. The secretion of serpentine was undetected during the first 24 h in all the samples. Treatment with the inhibitors quinidine and glibenclamide provoked a significant reduction of serpentine secretion in the hairy roots compared to the control. Based on our results, we found evidence that ABC transporters might participate in the secretion of MIAs by C. roseus hairy roots.
Downloads
References
Zhu, X.; Zeng, X.; Sun, C.; Chen, S. Front. Med. 2014. 8, 285-293.
Oudin, A.; Courtois, M.; Rideau, M.; Clastre, M. Phytochem. Rev. 2007, 6, 259-276.
Facchini, P. J.; De Luca, V. Plant. J. 2008, 54, 763-784.
Dugé de Bernonville, T.; Clastre, M.; Besseau, S.; Oudin, A.; Burlat, V.; Glevarec, G.; Lanoue, A.; Papon, N.; Giglioli-Guivarc’h, N.; St-Pierre, B.; Courdavault, V. Phytochemistry. 2015. 113, 9-23.
Deus-Neumann, B.; Zenk, M. H. Planta. 1984, 162, 250-260.
McCaskil, D. G.; Martin, D. L.; Scott, A. I. Plant Physiol. 1988, 87, 402-408
Neumann, D.; Krauss, G.; Hieke, M.; Groger, D. Planta Med. 1983, 48, 20-23.
Carqueijeiro, I.; Noronha, H.; Duarte, P.; Geros, H. V.; Sottomayor, M. Plant Physiol. 2013, 162, 1486-1496.
Pomahacova, B.; Dusek, J.; Duskova, J.; Yazaki, K.; Roytrakul, S.; Verpoorte, R. J. Plant Physiol. 2009, 166, 1405-1413.
El-Guizani, T.; Guibert, C.; Triki, S.; St-Pierre, B.; Ducos, E. J. Genet. 2014, 93, 21-33.
Loyola-Vargas, V. M.; Galaz-Avalos, R. M.; Rodríguez-Ku, J. R. Phytochem. Rev. 2007, 6, 307-339.
Roepke, J.; Salim, V.; Wu, M.; Thamm, A. M. K.; Murata, J.; Ploss, K.; Boland, W.; De Luca, V. Proc. Natl. Acad. Sci. (USA). 2010, 107, 15287-15292.
Yu, F.; De Luca, V. Proc. Natl. Acad. Sci. (USA). 2013, 110, 15830-15835.
Vázquez-Flota, F.; Moreno-Valenzuela, O. A.; Miranda-Ham, M. d. L.; Coello-Coello, J.; Loyola-Vargas, V. M. Plant Cell Tiss. Org. Cult. 1994, 38, 273-279.
Ruíz-May, E.; Galaz-Avalos, R. M.; Loyola-Vargas, V. M. Mol. Biotechnol. 2009, 41, 278-285.
Lee-Parsons, C. W. T.; Ertük, S. Plant Cell Rep. 2005, 24, 677- 682.
Godoy-Hernández, G.; Loyola-Vargas, V. M. Plant Cell Rep. 1997, 16, 287-290.
Loyola-Vargas, V. M.; Ruíz-May, E.; Galaz-Avalos, R. M.; De-la- Peña, C. Plant Signal. Behav. 2012, 7, 611-614.
Eichhorn, H.; Klinghammer, M.; Becht, P.; Tenhaken, R. J. Exp. Bot. 2006, 57, 2193-2201.
Loyola-Vargas, V. M.; Broeckling, C. D.; Badri, D. V.; Vivanco, J. M. Planta. 2007, 225, 301-310.
Sugiyama, A.; Shitan, N.; Yazaki, K. Plant Physiol. 2007, 144, 2000-2008.
Badri, D. V.; Loyola-Vargas, V. M.; Du, J.; Stermitz, F. R.; Broeckling, C. D.; Iglesias-Andreu, L. G.; Vivanco, J. M. New Phytol. 2008, 179, 209-223.
Baetz, U.; Martinoia, E. Trends Plant Sci. 2014, 19, 90-98.
De-la-Peña, C.; Loyola-Vargas, V. M. Plant Physiol. 2014, 166, 701-719.
Yamamoto, H.; Suzuki, M.; Suga, Y.; Fukui, H.; Tabata, M. Plant Cell Rep. 1987, 6, 356-359.
Yazaki, K. FEBS Lett. 2006, 580, 1183-1191.
Badri, D. V.; Loyola-Vargas, V. M.; Broeckling, C. D.; De-la- Peña, C.; Jasinski, M.; Santelia, D.; Martinoia, E.; Sumner, L. W.; Banta, L. M.; Stermitz, F. R.; Vivanco, J. M. Plant Physiol. 2008, 146, 762-771.
Yu, F.; De Luca, V. Transport of monoterpenoid indole alkaloids in Catharanthus roseus, Springer International Publishing, Plant ABC Transporters (Geisler, M.; ed.), Switzerland. 2014, 63-75.
Lew, R. R.; Spanswick, R. M. Plant Physiol. 1984, 75, 1-6.
Cantley, L. C., Jr.; Cantley, L. G.; Josephson, L. J. Biol. Chem. 1978, 253, 7361-7368.
Willsky, G. R.; White, D. A.; McCabe, B. C. J. Biol. Chem. 1984, 259, 13273-13281.
Horio, M.; Gottesman, M. M.; Pastan, I. Proc. Natl. Acad. Sci. (USA). 1988, 85, 3580-3584.
Urbatsch, I. L.; Sankaran, B.; Weber, J.; Senior, A. E. J. Biol. Chem. 1995, 270, 19383-19390.
Golstein, P. E.; Boom, A.; van Geffel, J.; Jacobs, P.; Masereel, B.; Beauwens, R. Pflüg. Arch. Eur. J. Phy. 1999, 437, 652-660.
Shitan, N.; Bazin, I.; Dan, K.; Obata, K.; Kigawa, K.; Ueda, K.; Sato, F.; Forestier, C.; Yazaki, K. Proc. Natl. Acad. Sci. (USA). 2003, 100, 751-756.
Palmgren, M. G.; Christensen, G. J. Biol. Chem. 1994, 269, 3027- 3033.
Taguchi, Y.; Yoshida, A.; Takada, Y.; Komano, T.; Ueda, K. FEBS Lett. 1997, 401, 11-14.
Schaller, A.; Oecking, C. Plant Cell. 1999, 11, 263-272.
Wigler, P. W. J. Bioenerg. Biomembr. 1996, 28, 279-284.
Boumendjel, A.; Baubichon-Cortay, H.; Trompier, D.; Perrotton, T.; Di Pietro, A. Med. Res. Rev. 2005, 25, 453-472.
Ueda, K.; Cardarelli, C.; Gottesman, M. M.; Pastan, I. Proc. Natl. Acad. Sci. (USA). 1987, 84, 3004-3008.
Sakai, K.; Shitan, N.; Sato, F.; Ueda, K.; Yazaki, K. J. Exp. Bot. 2002, 53, 1879-1886.
Terasaka, K.; Shitan, N.; Sato, F.; Maniwa, F.; Ueda, K.; Yazaki, K. Plant Cell Physiol. 2003, 44, 198-200.
Geisler, M.; Blakeslee, J. J.; Bouchard, R.; Lee, O. R.; Vincenzetti, V.; Bandyopadhyay, A.; Titapiwatanakun, B.; Peer, W. A.; Bailly, A.; Richards, E. L.; Ejendal, K. F. K.; Smith, A. P.; Baroux, C.; Grossniklaus, U.; Muller, A.; Hrycyna, C. A.; Dudler, R.; Murphy, A. S.; Martinoia, E. Plant J. 2005, 44, 179-194.
Kolaczkowski, M.; van der Rest, M.; Cybularz-Kolaczkowska, A.; Soumillion, J. P.; Konings, W. N.; Goffeau, A. J. Biol. Chem. 1996, 271, 31543-31548.
Goossens, A.; Hakkinen, S. T.; Laakso, I.; Oksman-Caldentey, K. M.; Inze, D. Plant Physiol. 2003, 131, 1161-1164.
Glavinas, H.; Krajesi, P.; Cserepes, J.; Sarkadi, B. Curr. Drug Del. 2004, 1, 27-42.
Jin, H.; Liu, D.; Zuo, K.; Gong, Y.; Miao, Z.; Chen, Y.; Ren, W.; Sun, X.; Tang, K. DNA Sequence. 2007, 18, 316-325.
Lee, E. K.; Kwon, M.; Ko, J.-H.; Yi, H.; Hwang, M. G.; Chang, S.; Cho, M. H. Plant Physiol. 2004, 134, 528-538.
Frangne, N.; Eggmann, T.; Koblischke, C.; Weissenbock, G.; Martinoia, E.; Klein, M. Plant Physiol. 2002, 128, 726-733.
Yazaki, K. Curr. Opi. Plant Biol. 2005, 8, 301-307.
Ciau-Uitz, R.; Miranda-Ham, M. d. L.; Coello-Coello, J.; Chí, B.; Pacheco, L. M.; Loyola-Vargas, V. M. In Vitro Cell. Dev. Biol. Plant. 1994, 30, 84-88.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Exp. Cell Res.,1968, 50, 151-158.
Yang, N. C.; Ho, W. M.; Chen, Y. H.; Hu, M. L. Anal. Biochem. 2002, 306, 323-327.
Liu, H.; Jiang, Y. M.; Luo, Y. B.; Jiang, W. B. Food Technol. Biotech. 2006, 44, 531-534.
Monforte-González, M.; Ayora-Talavera, T.; Maldonado-Mendoza, I. E.; Loyola-Vargas, V. M. Phytochem. Anal. 1992, 3, 117-121.
Sneath, P. H. A.; Sokal, R. R. Numerical taxonomy: the principles and practice of numerical classification, 1973.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
