Role of Different Transporting Systems in the Secretion of Alkaloids by Hairy Roots of Catharanthus roseus (L) G. Don
DOI:
https://doi.org/10.29356/jmcs.v59i2.24Keywords:
ABC transporters, alkaloids, Catharanthus roseus, exudation, inhibitors.Abstract
Knowledge on the biosynthetic pathways of the monoterpene alkaloids is enormous, but little is known about their mechanism of transporting system from the plant cell. There is not concrete evidence confirming the role of ABC transporters in the secretion of monoterpene indole alkaloids (MIAs) in Catharanthus roseus. Therefore, in order to determine the role of different transporting systems involved in the MIAs translocation, we employed a pharmacological approach by using transport inhibitors such as, KCN, Na3VO4, quinidine and glibenclamide in hairy root cultures of C. roseus. It was found that the accumulation of ATP drastically decreased in the presence of KCN or 100 μM acetylsalicylic acid (ASA)/100 μM KCN. The treatment with the inhibitors KCN and glibenclamide in the presence of ASA significantly increased the ajmalicine secretion compared to the control. The secretion of serpentine was undetected during the first 24 h in all the samples. Treatment with the inhibitors quinidine and glibenclamide provoked a significant reduction of serpentine secretion in the hairy roots compared to the control. Based on our results, we found evidence that ABC transporters might participate in the secretion of MIAs by C. roseus hairy roots.
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Zhu, X.; Zeng, X.; Sun, C.; Chen, S. Front. Med. 2014. 8, 285-293. DOI: https://doi.org/10.1007/s11684-014-0350-2
Oudin, A.; Courtois, M.; Rideau, M.; Clastre, M. Phytochem. Rev. 2007, 6, 259-276. DOI: https://doi.org/10.1007/s11101-006-9054-9
Facchini, P. J.; De Luca, V. Plant. J. 2008, 54, 763-784. DOI: https://doi.org/10.1111/j.1365-313X.2008.03438.x
Dugé de Bernonville, T.; Clastre, M.; Besseau, S.; Oudin, A.; Burlat, V.; Glevarec, G.; Lanoue, A.; Papon, N.; Giglioli-Guivarc’h, N.; St-Pierre, B.; Courdavault, V. Phytochemistry. 2015. 113, 9-23. DOI: https://doi.org/10.1016/j.phytochem.2014.07.023
Deus-Neumann, B.; Zenk, M. H. Planta. 1984, 162, 250-260. DOI: https://doi.org/10.1007/BF00397447
McCaskil, D. G.; Martin, D. L.; Scott, A. I. Plant Physiol. 1988, 87, 402-408 DOI: https://doi.org/10.1104/pp.87.2.402
Neumann, D.; Krauss, G.; Hieke, M.; Groger, D. Planta Med. 1983, 48, 20-23. DOI: https://doi.org/10.1055/s-2007-969871
Carqueijeiro, I.; Noronha, H.; Duarte, P.; Geros, H. V.; Sottomayor, M. Plant Physiol. 2013, 162, 1486-1496. DOI: https://doi.org/10.1104/pp.113.220558
Pomahacova, B.; Dusek, J.; Duskova, J.; Yazaki, K.; Roytrakul, S.; Verpoorte, R. J. Plant Physiol. 2009, 166, 1405-1413. DOI: https://doi.org/10.1016/j.jplph.2009.02.015
El-Guizani, T.; Guibert, C.; Triki, S.; St-Pierre, B.; Ducos, E. J. Genet. 2014, 93, 21-33. DOI: https://doi.org/10.1007/s12041-014-0327-6
Loyola-Vargas, V. M.; Galaz-Avalos, R. M.; Rodríguez-Ku, J. R. Phytochem. Rev. 2007, 6, 307-339. DOI: https://doi.org/10.1007/s11101-007-9064-2
Roepke, J.; Salim, V.; Wu, M.; Thamm, A. M. K.; Murata, J.; Ploss, K.; Boland, W.; De Luca, V. Proc. Natl. Acad. Sci. (USA). 2010, 107, 15287-15292. DOI: https://doi.org/10.1073/pnas.0911451107
Yu, F.; De Luca, V. Proc. Natl. Acad. Sci. (USA). 2013, 110, 15830-15835. DOI: https://doi.org/10.1073/pnas.1307504110
Vázquez-Flota, F.; Moreno-Valenzuela, O. A.; Miranda-Ham, M. d. L.; Coello-Coello, J.; Loyola-Vargas, V. M. Plant Cell Tiss. Org. Cult. 1994, 38, 273-279. DOI: https://doi.org/10.1007/978-94-011-0237-7_23
Ruíz-May, E.; Galaz-Avalos, R. M.; Loyola-Vargas, V. M. Mol. Biotechnol. 2009, 41, 278-285. DOI: https://doi.org/10.1007/s12033-008-9111-2
Lee-Parsons, C. W. T.; Ertük, S. Plant Cell Rep. 2005, 24, 677- 682. DOI: https://doi.org/10.1007/s00299-005-0026-0
Godoy-Hernández, G.; Loyola-Vargas, V. M. Plant Cell Rep. 1997, 16, 287-290. DOI: https://doi.org/10.1007/s002990050224
Loyola-Vargas, V. M.; Ruíz-May, E.; Galaz-Avalos, R. M.; De-la- Peña, C. Plant Signal. Behav. 2012, 7, 611-614. DOI: https://doi.org/10.4161/psb.20070
Eichhorn, H.; Klinghammer, M.; Becht, P.; Tenhaken, R. J. Exp. Bot. 2006, 57, 2193-2201. DOI: https://doi.org/10.1093/jxb/erj179
Loyola-Vargas, V. M.; Broeckling, C. D.; Badri, D. V.; Vivanco, J. M. Planta. 2007, 225, 301-310. DOI: https://doi.org/10.1007/s00425-006-0349-2
Sugiyama, A.; Shitan, N.; Yazaki, K. Plant Physiol. 2007, 144, 2000-2008. DOI: https://doi.org/10.1104/pp.107.096727
Badri, D. V.; Loyola-Vargas, V. M.; Du, J.; Stermitz, F. R.; Broeckling, C. D.; Iglesias-Andreu, L. G.; Vivanco, J. M. New Phytol. 2008, 179, 209-223. DOI: https://doi.org/10.1111/j.1469-8137.2008.02458.x
Baetz, U.; Martinoia, E. Trends Plant Sci. 2014, 19, 90-98. DOI: https://doi.org/10.1016/j.tplants.2013.11.006
De-la-Peña, C.; Loyola-Vargas, V. M. Plant Physiol. 2014, 166, 701-719. DOI: https://doi.org/10.1104/pp.114.241810
Yamamoto, H.; Suzuki, M.; Suga, Y.; Fukui, H.; Tabata, M. Plant Cell Rep. 1987, 6, 356-359. DOI: https://doi.org/10.1007/BF00269559
Yazaki, K. FEBS Lett. 2006, 580, 1183-1191. DOI: https://doi.org/10.1016/j.febslet.2005.12.009
Badri, D. V.; Loyola-Vargas, V. M.; Broeckling, C. D.; De-la- Peña, C.; Jasinski, M.; Santelia, D.; Martinoia, E.; Sumner, L. W.; Banta, L. M.; Stermitz, F. R.; Vivanco, J. M. Plant Physiol. 2008, 146, 762-771. DOI: https://doi.org/10.1104/pp.107.109587
Yu, F.; De Luca, V. Transport of monoterpenoid indole alkaloids in Catharanthus roseus, Springer International Publishing, Plant ABC Transporters (Geisler, M.; ed.), Switzerland. 2014, 63-75. DOI: https://doi.org/10.1007/978-3-319-06511-3_5
Lew, R. R.; Spanswick, R. M. Plant Physiol. 1984, 75, 1-6. DOI: https://doi.org/10.1104/pp.75.1.1
Cantley, L. C., Jr.; Cantley, L. G.; Josephson, L. J. Biol. Chem. 1978, 253, 7361-7368. DOI: https://doi.org/10.1016/S0021-9258(17)34509-X
Willsky, G. R.; White, D. A.; McCabe, B. C. J. Biol. Chem. 1984, 259, 13273-13281. DOI: https://doi.org/10.1016/S0021-9258(18)90689-7
Horio, M.; Gottesman, M. M.; Pastan, I. Proc. Natl. Acad. Sci. (USA). 1988, 85, 3580-3584. DOI: https://doi.org/10.1073/pnas.85.10.3580
Urbatsch, I. L.; Sankaran, B.; Weber, J.; Senior, A. E. J. Biol. Chem. 1995, 270, 19383-19390. DOI: https://doi.org/10.1074/jbc.270.33.19383
Golstein, P. E.; Boom, A.; van Geffel, J.; Jacobs, P.; Masereel, B.; Beauwens, R. Pflüg. Arch. Eur. J. Phy. 1999, 437, 652-660. DOI: https://doi.org/10.1007/s004240050829
Shitan, N.; Bazin, I.; Dan, K.; Obata, K.; Kigawa, K.; Ueda, K.; Sato, F.; Forestier, C.; Yazaki, K. Proc. Natl. Acad. Sci. (USA). 2003, 100, 751-756. DOI: https://doi.org/10.1073/pnas.0134257100
Palmgren, M. G.; Christensen, G. J. Biol. Chem. 1994, 269, 3027- 3033. DOI: https://doi.org/10.1016/S0021-9258(17)42042-4
Taguchi, Y.; Yoshida, A.; Takada, Y.; Komano, T.; Ueda, K. FEBS Lett. 1997, 401, 11-14. DOI: https://doi.org/10.1016/S0014-5793(96)01421-4
Schaller, A.; Oecking, C. Plant Cell. 1999, 11, 263-272. DOI: https://doi.org/10.1105/tpc.11.2.263
Wigler, P. W. J. Bioenerg. Biomembr. 1996, 28, 279-284. DOI: https://doi.org/10.1007/BF02110701
Boumendjel, A.; Baubichon-Cortay, H.; Trompier, D.; Perrotton, T.; Di Pietro, A. Med. Res. Rev. 2005, 25, 453-472. DOI: https://doi.org/10.1002/med.20032
Ueda, K.; Cardarelli, C.; Gottesman, M. M.; Pastan, I. Proc. Natl. Acad. Sci. (USA). 1987, 84, 3004-3008. DOI: https://doi.org/10.1073/pnas.84.9.3004
Sakai, K.; Shitan, N.; Sato, F.; Ueda, K.; Yazaki, K. J. Exp. Bot. 2002, 53, 1879-1886. DOI: https://doi.org/10.1093/jxb/erf052
Terasaka, K.; Shitan, N.; Sato, F.; Maniwa, F.; Ueda, K.; Yazaki, K. Plant Cell Physiol. 2003, 44, 198-200. DOI: https://doi.org/10.1093/pcp/pcg019
Geisler, M.; Blakeslee, J. J.; Bouchard, R.; Lee, O. R.; Vincenzetti, V.; Bandyopadhyay, A.; Titapiwatanakun, B.; Peer, W. A.; Bailly, A.; Richards, E. L.; Ejendal, K. F. K.; Smith, A. P.; Baroux, C.; Grossniklaus, U.; Muller, A.; Hrycyna, C. A.; Dudler, R.; Murphy, A. S.; Martinoia, E. Plant J. 2005, 44, 179-194. DOI: https://doi.org/10.1111/j.1365-313X.2005.02519.x
Kolaczkowski, M.; van der Rest, M.; Cybularz-Kolaczkowska, A.; Soumillion, J. P.; Konings, W. N.; Goffeau, A. J. Biol. Chem. 1996, 271, 31543-31548. DOI: https://doi.org/10.1074/jbc.271.49.31543
Goossens, A.; Hakkinen, S. T.; Laakso, I.; Oksman-Caldentey, K. M.; Inze, D. Plant Physiol. 2003, 131, 1161-1164. DOI: https://doi.org/10.1104/pp.102.016329
Glavinas, H.; Krajesi, P.; Cserepes, J.; Sarkadi, B. Curr. Drug Del. 2004, 1, 27-42. DOI: https://doi.org/10.2174/1567201043480036
Jin, H.; Liu, D.; Zuo, K.; Gong, Y.; Miao, Z.; Chen, Y.; Ren, W.; Sun, X.; Tang, K. DNA Sequence. 2007, 18, 316-325. DOI: https://doi.org/10.1080/10425170701207018
Lee, E. K.; Kwon, M.; Ko, J.-H.; Yi, H.; Hwang, M. G.; Chang, S.; Cho, M. H. Plant Physiol. 2004, 134, 528-538. DOI: https://doi.org/10.1104/pp.103.027045
Frangne, N.; Eggmann, T.; Koblischke, C.; Weissenbock, G.; Martinoia, E.; Klein, M. Plant Physiol. 2002, 128, 726-733. DOI: https://doi.org/10.1104/pp.010590
Yazaki, K. Curr. Opi. Plant Biol. 2005, 8, 301-307. DOI: https://doi.org/10.1016/j.pbi.2005.03.011
Ciau-Uitz, R.; Miranda-Ham, M. d. L.; Coello-Coello, J.; Chí, B.; Pacheco, L. M.; Loyola-Vargas, V. M. In Vitro Cell. Dev. Biol. Plant. 1994, 30, 84-88. DOI: https://doi.org/10.1007/BF02632126
Gamborg, O. L.; Miller, R. A.; Ojima, K. Exp. Cell Res.,1968, 50, 151-158. DOI: https://doi.org/10.1016/0014-4827(68)90403-5
Yang, N. C.; Ho, W. M.; Chen, Y. H.; Hu, M. L. Anal. Biochem. 2002, 306, 323-327. DOI: https://doi.org/10.1006/abio.2002.5698
Liu, H.; Jiang, Y. M.; Luo, Y. B.; Jiang, W. B. Food Technol. Biotech. 2006, 44, 531-534.
Monforte-González, M.; Ayora-Talavera, T.; Maldonado-Mendoza, I. E.; Loyola-Vargas, V. M. Phytochem. Anal. 1992, 3, 117-121. DOI: https://doi.org/10.1002/pca.2800030305
Sneath, P. H. A.; Sokal, R. R. Numerical taxonomy: the principles and practice of numerical classification, 1973.
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