GC/MS analysis and bioactive properties of extracts obtained from Clusia minor L. leaves


  • Raisa Mangas Marín Food and Pharmacy Institute (IFAL), University of Havana
  • Rodny Montes de Oca Porto Antidoping Laboratory, Institute of Sport Medicine
  • María Elizabeth Herrera Paredes Faculty of Chemical Sciences, University of Guayaquil
  • Adonis Bello Alarcón Faculty of Chemical Sciences, University of Guayaquil
  • Ivones Hernández Balmaseda Department of Pharmacology, Institute of Marine Sciences (ICIMAR)
  • Roberto Menéndez Soto del Valle Center of Neurosciences (CNEURO)
  • Miriam Teresa Paz Lopes Department of Pharmacology, Laboratory of Antitumor Substances, Biological Science Institute (ICB), Federal University Minas Gerais (UFMG)
  • Idania Rodeiro Guerra Department of Pharmacology, Institute of Marine Sciences (ICIMAR)




Clusia minor L., sterols, triterpenoids, antioxidant, anti-inflammatory, cytotoxicity


Clusia minor L. is traditionally used to treat many disorders that including pain and inflammation such as sores and warts. Four extracts from the leaves of plant were prepared: hexane (CMH-A), ethyl acetate (CMH-B), methanol (CMH-C) and ethanol (CMH-E) and the pharmacological (antioxidant and anti-inflammatory properties) and toxicity effects were examined. Previously, the main constituents from CMH-A extract was revealed. Here, we present the GC/MS analysis of CMH-B and CMH-C. Thirty three compounds were identified in the CMH-B extract and twenty seven compounds in the CMH-C. The presence of D-α-tocopherol and lupeol was relevant in both extracts. The only sterols identified were sitosterol and stigmasterol. All of them showed effective radical scavenger properties in the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay, being CMH-E extract the most promissory (IC50 = 10.25 µg/mL). CMH-A, C and E extracts, administered topically (0.5–4 mg per ear), significant reduced ear edema induced by croton oil at 4 mg per ear, meanwhile CMH-B that was be able to significant reduce the inflammation at the dose of 2 mg per ear. We evaluated also the cytotoxic activity of the extracts against kidney cells (BHK), colon cancer (CT26), endothelial cancer cells (EA.hy926) and breast cancer (4T1). CMH-B extract showed the most cytotoxicity effect, with IC50 values in the range of 32.01-203.5 µg/mL. In addition, no oral acute toxicity after mice exposure to Clusia minor L. extracts was observed. The results suggest Clusia minor L. may be a good potential source of new bioactive agents for developing medicinal agents.


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