Phytochemical Profile and Insecticidal Potential of Leaf Essential Oil of Psidium guajava Growing in North Central Nigeria
DOI:
https://doi.org/10.29356/jmcs.v69i2.2245Keywords:
Psidium, guajava, β-caryophyllene, insecticidal activityAbstract
Loss of stored maize grains via infestation by Sitophylus zeamais contributes to food insecurity. This necessitates the use of synthetic chemicals by farmers to curtail the pest. Unfortunately, the pesticides are toxic to humans and unsafe for the environment. Interestingly, some plants possess phytochemicals that exhibit insecticidal activity without these drawbacks. This activity is linked to the type of phytochemicals, whose presence depends on environmental conditions that change with time of collection of plant samples. It is on this basis that we investigated how time of harvest affects the phytochemicals and insecticidal activity of P. guajava leaf essential oils. To accomplish these, pulverized leaves (500 g) from 7.00am and 1.00pm harvests were hydrodistilled individually for three hours using a Clevenger set up. GC–MS technique was used to characterize the oils while contact toxicity bioassay was used to assess the insecticidal activity of the oils. The yields of essential oils obtained from the leaves were 0.27±0.015 and 0.24±0.018 (w/w)%. β-Caryophyllene (14.7 and 18.2%), α-guaiene (13.7 and 10.6%), α-selinene (10.9 and 12.9%), globulol (9.5 and 8.1%), caryophyllene oxide (7.8 and 7.0 %) and eucalyptol (5.6 and 5.8 %) existed in higher quantities in the GC-MS results. Both oils were active against S. zeamais with LT50 of 59.23 and 121.09 hours and the highest activity was recorded for the oil of afternoon harvest. The oil from afternoon harvest can therefore serves as a cheaper and more innocuous substitute to synthetic insecticide for S. zeamais management in stored maize.
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References
http://faostat.fao.org, accessed in March 2024.
Ojo, J. A.; Omoloye, A. A. J. Insects. 2014, 11, 1-5. DOI:10.1155/2015/429579. DOI: https://doi.org/10.1155/2015/429579
Goga, D. P.; Mutemerewa, S.; Moyo, G.; Neeley, D. J. Crop Prot. 1991, 10, 287–292. DOI: https://doi.org/10.1016/0261-2194(91)90007-E. DOI: https://doi.org/10.1016/0261-2194(91)90007-E
Muzemu, S.; Chitamba, J.; Mutetwa, B. Agric. For. Fish. 2013, 2, 196–201. DOI:10.11648/j.aff.20130205.13. DOI: https://doi.org/10.11648/j.aff.20130205.13
Ojumoola, O. A.; Adesiyun, A. A.; Usman, L. A. J. Trop. Agric. (Trinidad). 2018, 95, 211-217.
Iwu, M., in: Handbook of African medicinal plants, Vol. 2, Boca Raton CRC Press, London, 1993, 464.
Seo, J.; Lee, S.; Elam, M. L.; Johnson, S. A.; Kang, J.; Arjmandi, B. H. Food Sci. Nutr. 2004, 2, 174–180. DOI: https://doi.org/10.1002/fsn3.91. DOI: https://doi.org/10.1002/fsn3.91
Dakappa, S. S.; Adhikari, R.; Timilsina, S. S.; Sajjekhan, S. J. Drug. Deliv. Ther. 2013, 3, 162–168. DOI:10.22270/jddt.v3i2.404. DOI: https://doi.org/10.22270/jddt.v3i2.404
Arima, H.; Danno, G. Biosci. Biotechnol. Biochem. 2002, 66, 1727 - 1730. DOI: 10.1271/bbb.66.1727. DOI: https://doi.org/10.1271/bbb.66.1727
Rahim, N.; Gomes, D. J.; Watanabe, H.; Rahman, S. R.; Chomvarin, C.; Endtz, H. P.; Alam, M. Jpn. J. Infect. Dis. 2010, 63, 271–274. DOI: https://doi.org/10.7883/yoken.63.271
Sanches, N. R.; Aparício, D.; Cortez, G.; Schiavini, M. S.; Nakamura, C. V.; Prado, B. Braz. Arch. Biol. Technol. 2005, 48, 429–436. DOI: https://doi.org/10.1590/S1516-89132005000300014. DOI: https://doi.org/10.1590/S1516-89132005000300014
Mukhtar, H. M.; Ansari, S. H.; Bhat, Z. A.; Naved, T.; Singh, P. Die Pharmazie. 2006, 61, 725–727.
Khan, M. I. H.; Ahmad, J. A. J. Med. Plant Res. 1985, 23, 95–103. DOI: https://doi.org/10.5530/pj.2011.23.6. DOI: https://doi.org/10.3109/13880208509069009
Siani, A. C.; Souza, M. C.; Henriques, H. G. M. O.; Ramos, M. F. S. Pharm. Biol. 2013, 51, 881-887. DOI: https://doi.org/10.3109/13880209.2013.768675. DOI: https://doi.org/10.3109/13880209.2013.768675
Edwin, S.; Edwin, J.; Deb, S.; Gupta, S. Indian Drugs. 2007, 44, 395-397.
Zaka, S. M.; Zeng, X-N.; Holfors, P.; Beattie, G. A. C. Insect. Sci. 2010, 17, 39–45. DOI: https://doi.org/10.1111/j.1744-7917.2009.01271.x DOI: https://doi.org/10.1111/j.1744-7917.2009.01271.x
Barman, J. C.; Zeng, X. J. Zool. 2014, 46, 1117-1124. DOI: https://doi.org/10.1055/s-0034-1378094
Nwinyi, O. C.; Chinedu, N. S.; Ajani, O. J. Med. Plants Res. 2008, 2, 189-192.
Berdi, J.; Aszalos, M.; Mcntt, K. L., in: Handbook of antimicrobial compounds, Vol. 7, Boca Raton, Fld.: CRS press, London, 1981, 255.
Arain, A.; Sherazi, S.; Mahesar, S. Nat. Prod. Commun. 2019, 1-5.
Satyal, P.; Paudel, P.; Lamichhane, B.; Setzer, W. N. Am. J. Essent. Oils Nat. Prod. 2015, 3, 11–14.
Smith, R. M.; Oliveros-Belardo, L. Asian J. Pharm. 1977, 3, 5-9.
Ogunwande, I. A.; Olawore, N. O.; Adeleke, K. A.; Ekundayo, O.; Koenig, W. A. Flav. Frag. J. 2003, 18, 136 – 138. DOI: https://doi.org/10.1590/1519-6984.230533.
Khadhri, A.; El Mokni, R.; Almeida, C.; Nogueira, J. M. F.; Araújo, M. E. M. Ind. Crops Prod. 2014, 52, 29-31. DOI: https://doi.org/10.1016/j.indcrop.2013.10.018. DOI: https://doi.org/10.1016/j.indcrop.2013.10.018
Usman, L. A.; Watti, O.; Ismaeel, R. O.; Ojumoola, O. A. JOTCSA. 2016, 3, 1-18. DOI: https://doi.org/10.18596/jotcsa.98689 DOI: https://doi.org/10.18596/jotcsa.98689
Hudaib, M.; Speroni, E.; Di Pietra, A. M.; Cavrini, V. J. Pharma. Biomed. Anal. 2002, 29, 691–700. DOI: 10.1016/s0731-7085(02)00119-x DOI: https://doi.org/10.1016/S0731-7085(02)00119-X
McConkey, M. E.; Gershenzon, J.; Croteau, R. B. J. Plant Physiol. 2000, 122, 215-224. DOI: 10.1104/pp.122.1.215 DOI: https://doi.org/10.1104/pp.122.1.215
Pagoula, F.; Baser, K. H. C.; Kurkcuoglu, M. J. Essent. Oil Res. 2000, 12, 333-335. DOI:10.1080/10412905.2000.9699530 DOI: https://doi.org/10.1080/10412905.2000.9699530
British Pharmacopia, Vol. II, Her Majesty's Stationery Office, Atlantic House, London, 1980, 121.
Jennings, W.; Shibamoto, T., in: Qualitative analysis of flavor volatiles by gas chromatography, Vol., Academic press Rapid Manuscript Reproduction, New York, 1980, 213-252.
Joulain, D.; Koenig, W. A., in: The atlas of spectra data of sesquiterpene hydrocarbons, Vol. 1, EB Verlog, Hamburg, Germany, 1998, 135.
Ogunwande, I. A.; Olawore, N. O.; Adeleke, K. A.; Ekundayo, O.; Koenig, W. A. Flav. Frag. J. 2003, 18, 136 – 138. DOI: https://doi.org/10.1002/ffj.1175
Degenhardt, J.; Kollner, T. G.; Gershenzon, J. Phytochem. 2009, 70, 1621-1637. DOI: https://doi.org/10.1016/j.phytochem.2009.07.030. DOI: https://doi.org/10.1016/j.phytochem.2009.07.030
Ijima, Y.; Davidovich-Rikanati, R.; Fridman, E.; Gang, D. R.; Bar, E.; Lewinsohn, E.; Pichersky, E. Plant Physiol. 2004, 136, 3724-3736. DOI: 10.1104/pp.104.051318. DOI: https://doi.org/10.1104/pp.104.051318
Bohlmann, J.; Steel, C. L.; Croteau, R. J. Bio. Chem. 1997, 272, 21784 – 21792. DOI: 10.1074/jbc.272.35.21784. DOI: https://doi.org/10.1074/jbc.272.35.21784
Papachristos, D. P.; Stamopoulos, D. C. J. Stored Prod. Res. 2003, 39, 433-441. DOI: https://doi.org/10.1016/S0022-474X(02)00036-X. DOI: https://doi.org/10.1016/S0022-474X(02)00036-X
Kim, S. I.; Yoon, J. S.; Jung, J. W.; Hong, K. B.; Ahn, Y. J.; Kwon, H. W. J. Asia-Pacific Entomol. 2010, 13, 369–373. DOI: https://doi.org/10.1016/j.aspen.2010.06.011. DOI: https://doi.org/10.1016/j.aspen.2010.06.011
Koul, O.; Wali, S.; Dhaliwal, G. S. Biopestic. Int. 2008, 4, 63–84.
Ismail, M. Pharm. Biol. 2006, 44, 619–626. DOI: https://doi.org/10.1080/13880200600897544. DOI: https://doi.org/10.1080/13880200600897544
Pascual-Villabolobos, M. J.; Robledo, A. Biochem. Syst. Ecol. 1999, 27, 1–10. DOI: https://doi.org/10.1016/S0305-1978(98)00051-9. DOI: https://doi.org/10.1016/S0305-1978(98)00051-9
Chareonviriyaphap, T.; Nararak J.; Sathantriphop, S.; Kongmee, M.; Mahiou-Leddet, V.; Ollivier, E.; Manguin, S. Acta Trop. 2019, 197, 105030 DOI: https://doi.org/10.1016/j.actatropica.2019.05.021
Rajendran, S.; Sriranjini, V. J. Stored Prod. Res. 2008, 44, 126–135. DOI:10.1016/j.jspr.2007.08.003. DOI: https://doi.org/10.1016/j.jspr.2007.08.003
Priestley, C. M.; Williamson, E. M.; Wafford, K. A.; Sattelle, D. B. Br. J. Pharmacol. 2003, 140, 1363–1372. DOI: 10.1038/sj.bjp.0705542. DOI: https://doi.org/10.1038/sj.bjp.0705542
Kostyukovsky, M.; Rafaeli, A.; Gileadi, C.; Demchenko, N.; Shaaya, E. Pest Manag. Sci. 2002, 58, 1101–1106. DOI: 10.1002/ps.548. DOI: https://doi.org/10.1002/ps.548
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Copyright (c) 2025 Lamidi Ajao Usman, Etimbuk Daniel Akpan, Olusegun Adebayo Ojumoola, Ridwan Olanrewaju Ismaeel, Aliu Bola Simbiat
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