Application of Sodium Dodecyl Sulfate Coated Iron Oxide Magnetic Nanoparticles for the Extraction and Spectrofluorimetric Determination of Propranolol in Different Biological Samples


  • Ahad Bavili Tabrizi Tabriz University of Medical Sciences.
  • Nadereh Dehghani Teymurlouie Tabriz University of Medical Sciences Payame Noor University



Biological samples, Fe3O4 magnetic nanoparticles, Pro-pranolol, Solid phase extraction, Spectrofluorimetry.


A new analytical approach was developed involving mag-netic solid-phase extraction (MSPE) and spectrofluorimetric determi-nation of propranolol (PRO) in biological fluids. A urine or plasma sample was prepared and adjusted to pH 3-4, then PRO was quickly

extracted using Fe3O4 magnetic nanoparticles (MNPs) modified by the surfactant sodium dodecyl sulfate (SDS) and determined applying spectrofluorimetry. Experimental conditions, such as the amount of MNPs and SDS, pH value, standing time, desorption solvent and maximal extraction volume have been adjusted to optimize the extraction process and to obtain analytical characteristics of the method. Linearity was observed in the analyte’s concentration range of 2-75 ng mL-1 for both urine and plasma samples. The correlation coefficients (r) were higher than 0.99. The method showed good precision and accuracy, with intra- and inter-assay precisions of less than 5.0% at all concentrations. Standard addition recovery tests were carried out, and the recoveries ranged from 79.4% to 90.4%. The limits of detection and quantification were 0.85 and 2.80 ng mL-1, respectively, for urine and 0.74 and 2.43 ng mL-1, respectively, for plasma. The method was applied to the determination of PRO in human urine and plasma samples.


Download data is not yet available.

Author Biographies

Ahad Bavili Tabrizi, Tabriz University of Medical Sciences.

Associate Professor of Analytical Chemistry, Dept. of Medicinal Chemistry, Faculty of Pharmacy & Biotechnology Research Center.

Nadereh Dehghani Teymurlouie, Tabriz University of Medical Sciences Payame Noor University

Drug Applied Research Center.


Department of Chemistry.


Silva, L. C.; Trevisan, M. G.; Poppi, R. J.; Sena, M. M. Anal. Chim. Acta 2007, 595, 282-288.

Fernández-Sánchez, J. F.; Segura Carretero, A.; Cruces-Blanco, C.; Fernández-Gutiérrez, A. J. Pharm. Biomed. Anal. 2003, 31,


Partani, P. L.; Modhave, Y.; Gurule, S.; Khuroo, A.; Monif, T.J. Pharm. Biomed. Anal. 2009, 50, 966-976.

Kim, H. K.; Hong, J. H.; Park, M. S.; Kang, J. S.; Lee, M. H. Biomed. Chromatogr. 2001, 15, 539-545.

El-Saharty, Y.S. J. Pharm. Biomed. Anal. 2003, 33, 699-709.

Ismail, Z.; Abdul Wahab, M. S.; Abdul Rahman, A. R. Bur. J. Gen. Med. 2004, 1, 42-47.

Šatínský, D.; Serralheiro, H. S.; Solich, P.; Araújo, A. N.; Monte-negro, Maria C.B.S.M. Anal. Chim. Acta 2007, 600, 122-128.

Zhang, L.; Su, X.; Zhang, C.; Ouyang, L.; Xie, Q.; Ma, M.; Yao, S. Talanta 2010, 82, 984-992.

Walle, T.; Oatis, J. E.; Walle, U. K.; Knapp, D. R. Drug Metabol. Dispos. 1982, 10, 122-127.

do Carmo Borges, N. C.; Modesto Rigato, H.; de Oliveira, P. R.; Rubert Nogueira, D.; Agnaldo Moreno, R.; Luiz Dalmora, S. J. Liq. Chromatogr. & Rel. Technol. 2008, 31, 2927-2941.

Zhang, J.; Ding, L.; Wen, A.; Wu, F.; Sun, L.; Yang, L. Asian J. Pharm. Sci. 2009, 4, 169-177.

Berzas Nevado, J. J.; Rodríguez Flores, J.; Castaneda Penalvo, G.; Guzman Bernardo, F. J. Anal. Chim. Acta 2006, 559, 9-14.

Madrakian, T.; Afkhami, A.; Mohammadnejad, M. Talanta 2009, 78, 1051-1055.

Jouyban, A.; Sorouraddin, M. H.; Farajzadeh, M. A.; Somi, M. H.; Fazeli-Bakhtiyari, R. Talanta 2015, 134, 681-689.

Yamini, Y.; Faraji, M. J. Pharmaceut. Anal. 2014, 4, 279-285.

Sun, L.; Chen, L.; Sun, X.; Du, X.; Yue, Y.; He, D.; Xu, H.; Zeng, Q.; Wang, H.; Ding, L. Chemosphere 2009, 77, 1306-1312.

Bagheri, H.; Zandi, O.; Aghakhani, A. Anal. Chim. Acta 2012, 716, 61-65.

Lizhao, X.; Shi, Y.; Cai, Y.; Mou, S. Environ. Sci. Technol. 2008, 42, 1201-1206.

Zhao, X.; Shi, Y.; Wang, T.; Cai, Y.; Jiang, G. J. Chromatogr. A 2008, 1188, 140-147.

Sun, L.; Zhang, C.; Chen, L.; Liu, J.; Jin, H.; Xu, H.; Ding, L. Anal. Chim. Acta 2009, 638, 162-168.

Huang, X. X.; Lu, J. Y.; Lai, H. F.; Wei, L. Q. J. Chin. Chem. Soc. 2015, 62, 52-58.

Mollahosseini, A.; Toghroli, M.; Kamankesh, M. J. Sep. Sci. 2015, 38, 3750-3757.

Bavili Tabrizi, A.; Rashidi, M. R.; Ostadi, H. J. Braz. Chem. Soc. 2014, 25, 709-715.

Tavakoli, M.; Shemirani, F.; Hajimahmoodi, M. Food Anal. Meth-ods 2014, 7, 100-108.

Karimi, M. A.; Hatefi-Mehrjardi, A.; Mohadesi, A.; Mohammadi, S. Z.; Taghdiri, M.; Yarahmadi, J.; Mahmoodian, H.; Nezhad Kho-rasani, S. Iran. J. Anal. Chem. 2014, 1, 7-11.

Baghban, N.; Haji Shabani, A. M.; Dadfarnia, S. J. Chin. Chem. Soc., 2012, 59 (6), 782-787.

Faraji, M.; Yamini, Y.; Rezaee, M. Talanta 2010, 81, 831-836.

Bavili Tabrizi, A.; Naini, S.; Parnian, K.; Mohammadi, S.; Emami zad, F.; Pourtaghi Anvarian, S.; Abdollahi, A. Quim. Nova 2014, 37(7), 1182-1187.

Dominguez, A.; Fernandez, A.; Gonzalez, N.; Iglesias, E.; Monte-negro, L. J. Chem. Educ. 1997, 74, 1227-1231.

FDA, Center for Drug Evaluation and Research, US Department of Health and Human Services, Guidance for Industry, Bioanalyt-ical Method Validation, 2001.

Thompson, M.; Ellison, S. L. R.; Wood, R. Pure Appl. Chem. 2002, 74(5), 835-855.

Zhou, X.; Li, X.; Zeng, Z. J. Chromatogr. A 2006, 1104, 359-365.






Regular Articles