Chemical Composition, Antiradical, Antiproliferative, Proapoptotic and Arrest Cell Cycle Activities of Bursera linanoe Extracts
DOI:
https://doi.org/10.29356/jmcs.v70i1.2515Keywords:
Bursera linanoe, antiproliferative, MDA-MB-231, A549, apoptosis, arrest cell cycleAbstract
Abstract. Bursera linanoe is a tree widely distributed in the state of Guerrero. The extracts from several species of Bursera exhibit a wide range of biological activities, including antiproliferative, apoptotic, and cell-cycle arrest effects, as well as antioxidant properties. This study aimed to determine the phytochemical profile and biological activities of leaf extracts from Bursera linanoe. The hexane (EHBl) and dichloromethane (EDBl) extracts were analyzed by Gas Chromatography-Mass Spectrometry analysis and evaluated for their in vitro antiradical activity against DPPH• and ABTS•+ radicals. Cell cultures of Triple-Negative Breast Cancer (MDA-MB-231) and Human Lung Adenocarcinoma (A549) cells were used to assess antiproliferative activity by the MTT assay. The apoptosis induction and cell-cycle arrest were detected by Annexin/propidium iodide (PI) staining. The phytochemical analysis indicated that the major constituents of the EHBl and EDBl extracts are lupeol, τ-sitosterol, and β-amyrin. The EDBl was the most effective at reducing the ABTS•+ radical (IC50: 113.29 µg mL-1). In addition, proliferation of MDA-MB-231 and A549 cancer cells was significantly inhibited by EHBl, with IC50 values of 78.45 and 266.69 µg mL-1, respectively, and no evidence of toxicity against the non-tumor cell line MCF-10A. Additionally, EHBl extract (200 µg mL-1) increased the percentage of cells in apoptosis and in the G0/G1 phase, and arrested the cell cycle in cancer cell lines. In conclusion, this study demonstrates the biological activities of B. linanoe extracts, highlighting its pharmacological potential as a source of compounds for cancer treatment.
Resumen. Bursera linanoe es un árbol ampliamente distribuido en el estado de Guerrero. Los extractos de varias especies de Bursera presentan una amplia gama de actividades biológicas, incluyendo propiedades antiproliferativas, apoptóticas, de detención del ciclo celular y antioxidantes. El objetivo de este estudio fue determinar el perfil fitoquímico y las actividades biológicas de los extractos de hojas de Bursera linanoe. Los extractos de hexano (EHBl) y diclorometano (EDBl) se analizaron mediante cromatografía de gases con espectroscopía de masas y se evaluó su actividad antirradical in vitro frente a los radicales DPPH• y ABTS•+. El cultivo celular de células de cáncer de mama triple negativo (MDA-MB-231) y de adenocarcinoma de pulmón humano (A549) se utilizó para evaluar la actividad antiproliferativa mediante el ensayo de MTT. La inducción de apoptosis y la detención del ciclo celular se evaluaron mediante tinción con anexina/yoduro de propidio (PI). El análisis fitoquímico indicó que los principales constituyentes de los extractos de EHBl y EDBl son lupeol, τ-sitosterol y β-amirina. El EDBl fue el más eficaz para reducir el radical ABTS•+ (CI50: 113.29 µg mL-1). Además, la proliferación de las células cancerosas MDA-MB-231 y A549 se inhibió significativamente con EHBl, con CI50 de 78.45 y 266.69 µg mL-1, respectivamente, sin evidencia de toxicidad en las células no tumorales MCF-10A. Además, el extracto de EHBl (200 µg mL-1) aumentó el porcentaje de células en apoptosis y detuvo el ciclo celular en la fase G0/G1 en líneas celulares cancerosas. En conclusión, este estudio demuestra las actividades biológicas de los extractos de B. linanoe y evidencia su potencial farmacológico como fuente de compuestos naturales para el tratamiento del cáncer.
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