In vitro Anticoagulant Activity of Leaf Essential Oil of Artemisia absinthium. L on Human Blood

Maamri Sarra; Laoufi Razika; Belmecheri  Mohamed Reda; Djeziri Mourad; Khoukha Alileche

doi:10.29356/jmcs.v70i1.2569

In vitro Anticoagulant Activity of Leaf Essential Oil of Artemisia absinthium. L on Human Blood

Authors

Maamri Sarra ¹Université Amar Telidji, ²University M'hamed Bougara of Boumerdes https://orcid.org/0000-0002-7502-298X
Laoufi Razika University M'hamed Bougara of Boumerdes https://orcid.org/0000-0002-5424-0072
Belmecheri Mohamed Reda University M'hamed Bougara of Boumerdes
Djeziri Mourad University M’hamed Bougara of Boumerdes
Khoukha Alileche

DOI:

https://doi.org/10.29356/jmcs.v70i1.2569

Keywords:

Artemisia absinthium.L, essential oil, anticoagulant activity, Quick time, Cephalin kaolin time

Abstract

Abstract. The blood coagulation is a fast and efficient process that generates clot formation, which requires regulation. The blood coagulation derangement is a characteristic of many disease conditions. This study investigated the in vitro effects of leaf essential oil extract of Artemisia absinthium L. on blood coagulation. Essential oil was obtained by hydrodistillation. Then the phytochemical characterization of the essential oil was done by gas chromatography-mass spectrometry (GC-MS). Varying concentrations (1/10–1/40 v/v) of the essential oil were tested in vitro on blood coagulation profile; Quick time (QT) and Cephalin kaolin time (CKT). The leaf essential oil was characterized by a phytochemical profile dominated by β-thujene and chamazulene, among other monoterpenic and sesquiterpenic constituents. The essential oil significantly prolonged both Quick time (QT) and Cephalin-kaolin time (CKT), indicating a marked interference with both extrinsic and intrinsic coagulation pathways.

These findings suggest a broad anticoagulant effect, likely mediated through the combined action of bioactive terpenoids affecting platelet function, coagulation cascade enzymes, and/or membrane-associated signaling processes. Overall, the essential oil contains bioactive compounds with potential anticoagulant properties, supporting its possible therapeutic relevance in the management of blood coagulation disorders.

Resumen. La coagulación sanguínea es un proceso rápido y eficiente de formación de coágulos, el cual requiere regulación. El desorden en la coagulación es una característica de muchas enfermedades. El presente estudio investigó los efectos in vitro del aceite esencial de las hojas de Artemisia absinthium L. El aceite esencial fue obtenido por hidrodestilación y la caracterización química fue realizada por cromatografía de gases – espectrometría de masas (CG-EM). Se probaron varias concentraciones (1/10–1/40 v/v) del aceite esencial in vitro en el perfil de la coagulación sanguínea por tiempo de respuesta rápida (QT) y por tiempo de cefalina con kaolina (CKT). El aceite esencial de las hojas se caracterizó por un perfil fitoquímico dominado por β-thujeno y chamazuleno, entre otros constituyentes monoterpénicos y sesquiterpénicos. El aceite esencial prolongó significativamente los tiempos QT y CKT, indicando una interferencia marcada con las rutas de coagulación intrínsecas y extrínsecas. Estos hallazgos indican un efecto anticoagulante amplio, mediado posiblemente por la acción combinada de terpenoides bioactivos que afectan a la función de las plaquetas, a las enzimas de la cascada de coagulación, y/o a procesos de señalización asociados con la membrana. En general, el aceite esencial contiene principios bioactivos con propiedades anticoagulantes, lo cual da cuenta de su posible relevancia terapéutica en el manejo de los desórdenes de coagulación sanguínea.

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Author Biographies

Maamri Sarra, ¹Université Amar Telidji, ²University M'hamed Bougara of Boumerdes

¹Laboratoire des Sciences Fondamentales

²Laboratory of Technologies, Soft and Valorization of Biological Materials and Biodiversity- Faculty of Science

Laoufi Razika, University M'hamed Bougara of Boumerdes

Laboratory of Technologies, Soft and Valorization of Biological Materials and Biodiversity- Faculty of Science

Belmecheri Mohamed Reda, University M'hamed Bougara of Boumerdes

Laboratory of Hydrocarbon Technology. Faculty of Hydrocarbons and Chemistry

Djeziri Mourad, University M’hamed Bougara of Boumerdes

Research Laboratory in Food Technology, Faculty of Technology

Center for Scientific and Technical Research in Physico-Chemical Analysis

References

1. Benjamin, E. J.; Blaha, M. J.; Chiuve, S. E.; Cushman, M.; Das, S. R.; Deo, R.; Floyd, J.; Fornage, M.; Gillespie, C.; Isasi, C. Circulation. 2017, 135, e146–e603. DOI: https://doi.org/10.1161/CIR.0000000000000485

2. Bowles, L.; Platton, S.; Yartey, N.; Dave, M.; Lee, K.; Hart, D. P.; MacDonald, V.; Green, L.; Sivapalaratnam, S.; Pasi, K. J. N. Engl. J. Med. 2020, 383, 288–290. DOI: https://doi.org/10.1056/NEJMc2013656

3. Giannis, D.; Ziogas, I. A.; Gianni, P. J. Clin. Virol. 2020, 127, 104362. DOI: https://doi.org/10.1016/j.jcv.2020.104362

4. Nguyen, T. V. A.; Ly, H. D. T.; Vu, T. T.; Nguyen, T. T.; Le, H. L. Asian J. Pharmacogn. 2018, 2, 30–31.

5. Hirsh, J.; O’Donnell, M.; Weitz, J. I. Blood. 2005, 105, 453–463. DOI : https:// doi: 10.1182/blood-2003-12-4195

6. Koenig-Oberhuber, V.; Filipovic, M. Br. J. Anaesth. 2016, 117(Suppl 2), ii74–ii84. DOI:https:// 10.1093/bja/aew214

7. Tognolini, M.; Barocelli, E.; Ballabeni, V.; Bruni, R.; Bianchi, A.; Chiavarini, M.; Impicciatore, M. Life Sci. 2006, 78, 1419–1432. DOI: https://doi.org/10.1016/j.lfs.2005.07.020

8. Hou, Y.; Li, H.; Zhu, L.; Li, Y.; Zeng, Y.; Quan, T.; Xiang, Z.; Zhang, Y.; Bian, Y.; Wei, Y. Front. Pharmacol. 2025, 16, 1537776. DOI: https://doi.org/10.3389/fphar.2025.1537776

9. Kim, K.; Park, K.-I. Evid.-Based Complement. Altern. Med. 2019, 7125162. DOI: https://doi.org/10.1155/2019/7125162

10. Chua, T.; Koh, H. Mini-Rev. Med. Chem. 2006, 6, 611–624. DOI: https://doi.org/10.2174/138955706777435751

11. Nikitina, L. E.; Pavelyev, R. S.; Gilfanov, I. R.; Kiselev, S. V.; Azizova, Z. R.; Ksenofontov, A. A.; Bocharov, P. S.; Antina, E. V.; Klochkov, V. V.; Timerova, A. F.; Rakhmatullin, I. Z.; Ostolopovskaya, O. V.; Khelkhal, M. A.; Boichuk, S. V.; Galembikova, A. R.; Andriutsa, N. S.; Frolova, L. L.; Kutchin, A. V.; Kayumov, A. R. Bioengineering. 2022, 9, 1. DOI: https://doi.org/10.3390/bioengineering9010001

12. Ashwini, U.; Asha, S. Int. J. Pharm. Clin. Res. 2017, 9, 425–429. DOI: https://doi.org/10.25258/ijpcr.v9i5.8607

13. Batiha, G. E.; Olatunde, A.; El-Mleeh, A.; Hetta, H. F.; Al-Rejaie, S.; Alghamdi, S.; Zahoor, M.; Beshbishy, A. M. ; Murata, T.; Zaragoza-Bastida, A.; Rivero-Pérez, N. Antibiotics. 2020, 9, 353. DOI: https://doi.org/10.3390/antibiotics9060353

14. Msaada, K.; Salem, N.; Bachrouch, O.; Bousselmi, S.; Tammar, S.; Alfaify, A.; Marzouk, B. J. Chem. 2015, 804658. DOI: https://doi.org/10.1155/2015/804658

15. Mohammadian, A.; Moradkhani, S.; Ataei, S.; Shayesteh, T. H.; Sedaghat, M.; Kheiripour, N.; Ranjbar, A. J. HerbMed Pharmacol. 2016, 5, 29–32.

16. Diab M. K.; Abu-Elsaoud A. M.; Salama M. G.; Ghareeb E. M. Phytochem. Rev. 2026, 25, 1225–1262. DOI: https://doi.org/10.1007/s11101-025-10163-0

17. Ahmad, F.; Khan, R. A.; Rasheed, S. J. Islam. Acad. Sci. 1992, 5, 111–114.

18. Hadi, A.; Nazari, H.; Parvini, S.; Nazari, N.; Mohammadi, A. Int. J. Pharm. Sci. Rev. Res. 2014, 24, 237–244.

19. Basiri, Z.; Zeraati, F.; Esna-Ashari, F.; Mohammadi, F.; Razzaghi, K.; Araghchian, M.; Moradkhani, S. Iran J. Med. Sci. 2017, 42, 524–531.

20. Higashi, Y. Antioxidants. 2022, 11, 1958. DOI: https://doi.org/10.3390/antiox11101958

21. Zhang, C. Y.; Liu, S.; Yang, M. World J. Hepatol. 2023, 15, 180–200. DOI: https://doi.org/10.4254/wjh.v15.i2.180

22. Kaiser, R.; et al. Front. Cardiovasc. Med. 2022, 8, 824112.DOI: https://doi.org/10.3389/fcvm.2021.824112

23. Association Française de Normalisation (AFNOR). Huiles Essentielles: Tome 1, Échantillonnage et Méthodes d’Analyse, 6th ed.; AFNOR: Paris La Défense, France, 2000, 471.

24. Bouchenak, F.; Degaichia, H.; Lamgharbi, A.; Benrebiha, F. Agrobiologia. 2018, 8, 886–895.

25. Djeridane, A.; Yousfi, M.; Nadjemi, B.; Maamri, S.; Djireb, F.; Stocker, P. J. Enzyme Inhib. Med. Chem. 2006, 21, 719–726. DOI: https://doi.org/10.1080/14756360600810399

26. Mourão, P. A.; Pereira, M. S.; Pavão, M. S.; Mulloy, B.; Tollefsen, D. M.; Mowinckel, M.-C.; Abildgaard, U. J. Biol. Chem. 1996, 271, 23973–23984. DOI: https://doi.org/10.1074/jbc.271.39.23973

27. Qi, X.; Mao, W.; Gao, Y.; Chen, Y.; Zhao, C.; Li, N.; Wang, C.; Yan, M.; Lin, C. Carbohydr. Polym. 2012, 90, 1804–1810. DOI: https://doi.org/10.1016/j.carbpol.2012.07.077

28. Mutlucan, M.. Turk. J. Field Crops 2026, 31, 1 https://doi.org/10.17557/tjfc.1878799

29. Tenscher, E.; Anton, R.; Lobstein, A., in : Plantes Aromatiques: Épices, Aromates, Condiments et Huiles Essentielles; Tec & Doc: Paris, France, 2005, 3-50, 121-124.

30. Lopes-Lutz, D.; Alviano, D. S.; Alviano, C. S.; Kolodziejczyk, P. P. Phytochemistry. 2008, 69, 1732–1738. DOI: https://doi.org/10.1016/j.phytochem.2008.02.014

31. Derwich, E.; Benziane, Z.; Boukir, A. J. Environ. Agric. Food Chem. 2009, 8, 1202–1211.

32. Riahi, L.; Chograni, H.; Elferchichi, M.; Zaouali, Y.; Zoghlami, N.; Mliki, A. Ind. Crops Prod. 2013, 46, 290–296. DOI: https://doi.org/10.1016/j.indcrop.2013.01.036

33. Sallé, J.-L.; Pelletier, J., in: Les Huiles Essentielles; Frison-Roche: Paris, France, 1991.

34. Flück, H.; Weitzel, R., in: Nos Plantes Médicinales; Payot: Paris, France, 1942.

35. Kordali, S.; Kotan, R.; Mavi, A.; Cakir, A.; Ala, A.; Yildirim, A. J. Agric. Food Chem. 2005, 53, 9452–9458. DOI: https://doi.org/10.1021/jf0516538

36. Taherkhani, M.; Rustaiyan, A.; Rasooli, I.; Taherkhani, T. Afr. J. Pharm. Pharmacol. 2013, 7, 30–36. DOI: https://doi.org/10.5897/AJPP12.945

37. Judzentiene, A.; Mockutė, D. Chemija. 2004, 15, 64–68.

38. Patočka, J.; Plucar, B. J. Appl. Biomed. 2003, 1, 199–205.

39. Mohammadi, A.; Sani, T. A.; Ameri, A.; Imani, M.; Golmakani, E.; Kamali, H. Pharmacogn. Res. 2014, 7, 329–334. DOI: https://doi.org/10.4103/0974-8490.158441

40. Srinivasan, R.; Aruna, A.; Lee, J. S.; Kim, M.; Shivakumar, M. S.; Natarajan, D. Biomed. Res. Int. 2020, 8716927. DOI: https://doi.org/10.1155/2020/8716927

41. Moro, M. A.; Russell, R. J.; Cellek, S.; Lizasoain, I.; Su, Y.; Darley-Usmar, V. M.; Radomski, M. W.; Moncada, S. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 1480–1485. DOI: https://doi.org/10.1073/pnas.93.4.1480

42. Zhang, G.; Xiang, B.; Dong, A.; Skoda, R. C.; Daugherty, A.; Smyth, S. S.; Du, X.; Li, Z. Blood. 2011, 118, 3670–3679. DOI: https://doi.org/10.1182/blood-2011-03-341107

43. Loscalzo, J. Circ. Res. 2001, 88, 756–762. DOI: https://doi.org/10.1161/hh0801.089861

44. Safayhi, H.; Sabieraj, J.; Sailer, E. R.; Ammon, H. P. Planta Med. 1994, 60, 410–413. DOI: https://doi.org/10.1055/s-2006-959520

45. Zhao, Y.; Wang, W.; Wang, Q.; Zhang, X.; Ye, L. Biochem. Biophys. Res. Commun. 2012, 418, 647–651. DOI: https://doi.org/10.1016/j.bbrc.2012.01.068

46. Moilanen, E.; Kirkkola, A. L.; Kankaanranta, H.; Nieminen, M. M.; Vapaatalo, H. Inflammation. 1993, 17, 705–714.

47. Rainger, G. E.; Chimen, M.; Harrison, M. J.; Yates, C. M.; Harrison, P.; Watson, S. P.; Lordkipanidzé, M.; Nash, G. B. Platelets. 2015, 26, 507–520. DOI: https://doi.org/10.3109/09537104.2015.1064881

48. Rayes, J.; Bourne, J. H.; Brill, A.; Watson, S. P. Res. Pract. Thromb. Haemost. 2020, 4, 23–35. DOI: https://doi.org/10.1002/rth2.12266

49. DeFilippis, A. P.; Rai, S. N.; Cambon, A.; Miles, R. J.; Jaffe, A. S.; Moser, A. B.; Jones, R. O.; Bolli, R.; Schulman, S. P. Nutr. Metab. Cardiovasc. Dis. 2014, 24, 428–433. DOI: https://doi.org/10.1016/j.numecd.2013.08.012

50. Yang, N. Y.; et al. Fitoterapia. 2011, 82, 692–695. DOI: https://doi.org/10.1016/j.fitote.2011.02.007

51. Souza, R. P.; Pimentel, V. D.; de Sousa, R. W. R.; Sena, E. P.; da Silva, A. C. A.; Dittz, D.; Ferreira, P. M. P.; de Oliveira, A. P. Naunyn-Schmiedeberg’s Arch. Pharmacol. 2024, 397, 8145–8160. DOI: https://doi.org/10.1007/s00210-024-03173-w

52. Salehi, B.; Upadhyay, S.; Erdogan Orhan, I.; Jugran, A. K.; Dias, D. A.; Dejam, M.; Sharopov, F.; Taheri, Y.; Martins, N.; Baghalpour, N.; Cho, W. C.; Sharifi-Rad, J. Biomolecules. 2019, 9, 1–20. DOI: https://doi.org/10.3390/biom9110522

53. Sebin, S. O.; Gulakar, B.; Tanyeli, A.; Guler, M. C.; Erbas, E.; Baser, S.; Celebi, D. Toxicon. 2026, 269, 108637. DOI: https://doi.org/10.1016/j.toxicon.2025.108637

54. de Cássia da Silveira e Sá, R.; Andrade, L. N.; de Sousa, D. P. Molecules. 2013, 18, 1227–1254. DOI: https://doi.org/10.3390/molecules18011227

55. Alberti, T.B.; Barbosa, W.L.R.; Vieira, J.L.F.; Raposo, N.R.B.; Dutra, R.C. Int. J. Mol. Sci. 2017, 18, 691. DOI: https://doi.org/10.3390/ijms18040691

56. Cheng, Y.; Dong, Z.; Liu, S. Pharmacology. 2014, 94, 1–12. DOI: https://doi.org/10.1159/000362689

57. Irrera, N.; D’Ascola, A.; Pallio, G.; Bitto, A.; Mannino, F.; Arcoraci, V.; Rottura, M.; Ieni, A.; Minutoli, L.; Metro, D.; Vaccaro, M.; Altavilla, D.; Squadrito, F. Cancers (Basel). 2020, 12, 1038. DOI: https://doi.org/10.3390/cancers12041038

58. Grover, S. P.; Mackman, N. Thromb. Vasc. Biol. 2019, 39, 331–338. DOI:https://doi.org/10.3390/molecules18011227

59. Yap, E. S.; Lijfering, W. M.; Timp, J. F.; Rosendaal, F. R.; Cannegieter, S. C.; Scheres, L. J. J. EJHaem. 2023, 4, 3–12. DOI: https://doi.org/10.1002/jha2.618Digital

60. Seo, M. J.; Kim, S. J.; Kang, T. H.; Rim, H. K.; Jeong, H. J.; Um, J. Y.; Hong, S. H.; Kim, H. M. Int. J. Immunopathol. Pharmacol. 2011, 24 (Suppl. 2), 33–39. DOI: https://doi.org/10.3109/08923973.2010.491082

61. de Sousa, M. A. R.; dos Santos Teixeira, G.; Marquesa, R. B.; de Sousa, L. M. R.; Ramos, R. M.; de França Bento, R. R.; Neto, B. C. V.; Gusmao, S. B. S.; Sá, J. L. S.; Maia Filho, A. L. M.M. A. R.; et al. S. Afr. J. Bot. 2024, 169, 341–349. DOI: https://doi.org/10.1016/j.sajb.2024.04.023

62. Tisserand, R.; Young, R., in: Essential Oil Safety: A Guide for Health Care Professionals, 2nd ed.; Elsevier Health Sciences: Amsterdam, The Netherlands, 2013.

63. Skalski, B.; Pawelec, S.; Jedrejek, D.; Rolnik, A.; Pietukhov, R.; Piwowarczyk, R.; Stochmal, A.; Olas, B. Biomed. Pharmacother. 2021, 139, 111618. DOI: https://doi.org/10.1016/j.biopha.2021.111618

64. Harrison, S.; Vavken, P.; Kevy, S.; Jacobson, M.; Zurakowski, D.; Murray, M. M. Am. J. Sports Med. 2011, 39, 729–734. DOI: https://doi.org/10.1177/0363546511401576

65. Chang, Y.; et al. Food Sci. Nutr. Res. 2025, 8, 1–5.

66. Oury, C.; Toth-Zsamboki, E.; Vermylen, J.; Hoylaerts, M. F. Blood. 2002, 100, 2499–2505. DOI: https://doi.org/10.1182/blood-2002-03-0812

67. Basavegowda, N.; Baek, K. H. Biomolecules. 2021, 11, 1267. DOI: https://doi.org/10.3390/biom11020244

68. Bunse, M.; Daniels, R.; Gründemann, C.; Heilmann, J.; Kammerer, D. R.; Keusgen, M.; Lindequist, U.; Melzig, M. F.; Morlock, G. E.; Schulz, H.; Schweiggert, R.; Simon, M.; Stintzing, F. C.; Wink, M. Front. Pharmacol. 2022, 13, 956541. DOI: https://doi.org/10.3389/fphar.2022.956541

69. Khodaei, N.; Houde, M.; Bayen, S.; Karboune, S.J. Food Sci. Technol. 2023, 60, 679–691. DOI: https://doi.org/10.1007/s13197-022-05653-4

70. Sobotková, A.; Mášová-Chrastinová, L.; Suttnar, J.; Štikarová, J.; Májek, P.; Reicheltová, Z.; Kotlín, R.; Weisel, J. W.; Malý, M.; Dyr, J. E. Free Radic. Biol. Med. 2009, 47, 1707–1714. DOI: https://doi.org/10.1016/j.freeradbiomed.2009.09.015