Single point calibration for quantitative speciation of selenomethionine in yeast Saccharomyces cerevisiae by HPLC-ICP-MS: using reliable, traceable and comparable measurements

Márcia Silva da Rocha, Lilian da Silva, Rodrigo Caciano de Sena, Thiago de Oliveira Araújo, Marcelo Dominguez de Almeida, María Luisa Fernández Sánchez, Alfredo Sanz Medel


The production of selenium and selenomethionine certified reference material in yeast (Saccharomyces cerevisiae) and certification studies for its homogeneity, stability and characterization, have been accomplished before in our laboratory. Based on such results and experience of our laboratory in Metrology and according to literature, we discuss here the advantages and limitations of the calibration by “single point” as an alternative method to the classical calibration curve. The method traceability to the SI is checked in order to secure a robust determination. As the model analyte the mass fraction of the Se species selenomethionine is measured and compared in yeast samples. Results showed that a good agreement was obtained by both methods (the reference one of the calibration curve and “single point calibration”). This simple approach can be used for routine control of selenomethionine in commercial supplements of yeast samples, but the work carried out demonstrated its potential for the general routine quantitative analysis of the possible and important selenium species in other Se-enriched supplements commercialised in Latin America (i.e. mushrooms, garlic etc).


selenomethionine; calibration curve; single point calibration; measurement uncertainty; HPLC-ICP-MS

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MacFarquhar, J. K.; Broussard, D. L.; Melstrom, P.; Hutchinson, R.; Wolkin, A.; Martin, C.; Burk, R. F.; Dunn, J. R.; Green, A. L.; Hammond, R.; Schaffner, W.; Jones, T. F.; Arch Intern Med 2010, 170, 256-261.

Aldosary, B. M.; Sutter, M. E.; Schwartz, M.; Morgan, B. W. Clin Toxicol 2012, 50, 57-64.

Arnaudguilhem C.; Bierla K., Ouerdane L.; Preud'homme H.; Yiannikouris A.; Lobinski R. Anal. Chim. Acta, 2012, 757, 26-38.

National Health Surveillance Agency (ANVISA) ; Accessed in November 2017.

Iyengar, V. J Radioanal Nucl Chem 2013, 297, 451-455.

Iyengar, V. J Food Compost Anal 2007, 20, 449-450.

Ting, T.; Sin, D. W.; Ho, C.; Chung, W. Accred Qual Assur 2006, 11, 172-174.

International Vocabulary of Metrology – Basic and General Concepts and Associated Terms International Vocabulary of Metrology, 2012.

Souza, S. V. C.; Junqueira, R. G. Anal. Chim. Acta 2005, 552, 25-35.

De Beer, J. O.; Naert, C. Accred Qual Assur 2012, 17, 265-274.

Nascimento, R. S.; Froes, R. E. S.; Silva, N. O. C.; Naveira, R. L. P.; Mendes, D. B. C.; Neto, W. B.; Silva, J. B. B. Talanta 2010, 80, 1102-1109.

Ribeiro, F. A. L.; Ferreira, M. M. C.; Morano, S. C.; Silva, L. R.; Schneider, R. P. Química Nova 2008, 31, 164-171.

Mulholland, M.; Hibbert, D. B. J. Chromatogr. A 1997, 762, 73-82.

Riu, J.; Rius, F. X. J. Chemometrics 1995, 9, 343-362.

ISO Guide 33 - Reference materials — Good practice in using reference materials, 2015.

Bánfai, B.; Kemény, S. Chemometrics 2012, 26, 117-124

JCGM 100:2008 - Evaluation of measurement data — Guide to the expression of uncertainty in measurement, 2008.

EURACHEM/CITAC Guide CG 4 Quantifying Uncertainty in Analytical Measurement, Third Edition, 2012.

Couto, P. R. G. Nota Técnica, DIMEC, nt-02/v.00, 2008.

Silva, L.; Souza, J. R.; Sánchez, M. L. F.; Araújo, T. O.; Rocha, M. S. Br J Anal Chem 2013, 12, 499–508.

Mermet, J. Spectrochim. Acta Part B 2010, 65, 509-523.

Huber, W. Accred. Qual. Assur. 2004, 9, 726.

Miller, J. N.; Analyst 1991, 116, 3-14.

Shapiro, S. S.; Wilk, M. B. Biometrika 1965, 52, 591-611.

Durbin, J.; Watson, G. S. Biometrika, 1951, 38, 159-177.

Féménias, J. L. J. Mol. Spectrosc 2003, 217, 32–42.

Féménias, J. L. J. Mol. Spectrosc 2004, 224, 73–98.

González, A. G; Herrador, M. A.; Asuero, A. G.; Sayago, A. Accred Qual Assur 2006, 11, 256–258.

Cuadros-Rodríguez, L.; Bagur-González, M. G.; Sánchez-Viñas, M.; González-Casado, A.; Gómez-Sáez, A. M. J. Chromatogr. A 2007, 1158, 33-46.

Linsinger, T. Application Note 1 - Comparison of a measurement result with the certified value European Commission - Joint Research Centre Institute for Reference Materials and Measurements (IRMM), 2010, Belgium.



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