Single point calibration for quantitative speciation of selenomethionine in yeast Saccharomyces cerevisiae by HPLC-ICP-MS: using reliable, traceable and comparable measurements

Authors

  • Márcia Silva da Rocha Inmetro
  • Lilian da Silva Inmetro
  • Rodrigo Caciano de Sena Inmetro
  • Thiago de Oliveira Araújo Inmetro
  • Marcelo Dominguez de Almeida Inmetro
  • María Luisa Fernández Sánchez Universidad de Oviedo
  • Alfredo Sanz Medel Universidad de Oviedo

DOI:

https://doi.org/10.29356/jmcs.v62i2.471

Keywords:

selenomethionine, calibration curve, single point calibration, measurement uncertainty, HPLC-ICP-MS

Abstract

The production of selenium and selenomethionine certified reference material in yeast (Saccharomyces cerevisiae) and certification studies for its homogeneity, stability and characterization, have been accomplished before in our laboratory. Based on such results and experience of our laboratory in Metrology and according to literature, we discuss here the advantages and limitations of the calibration by “single point” as an alternative method to the classical calibration curve. The method traceability to the SI is checked in order to secure a robust determination. As the model analyte the mass fraction of the Se species selenomethionine is measured and compared in yeast samples. Results showed that a good agreement was obtained by both methods (the reference one of the calibration curve and “single point calibration”). This simple approach can be used for routine control of selenomethionine in commercial supplements of yeast samples, but the work carried out demonstrated its potential for the general routine quantitative analysis of the possible and important selenium species in other Se-enriched supplements commercialised in Latin America (i.e. mushrooms, garlic etc).

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Author Biographies

Márcia Silva da Rocha, Inmetro

Researcher 

 National Institute of Metrology, Quality and Technology (Inmetro)Chemical Metrology Division – Dquim/Inorganic Analysis Laboratory – LabinAv Nossa Sra das Graças, 50. Duque de Caxias, RJ, Brazil 25250-020.

Phone: (55) 21 26799579      Fax: (55) 21 26799069

Lilian da Silva, Inmetro

Researcher 

 National Institute of Metrology, Quality and Technology (Inmetro)Chemical Metrology Division – Dquim/Inorganic Analysis Laboratory – LabinAv Nossa Sra das Graças, 50. Duque de Caxias, RJ, Brazil 25250-020.

Rodrigo Caciano de Sena, Inmetro

Researcher 

 National Institute of Metrology, Quality and Technology (Inmetro)Chemical Metrology Division – Dquim/Inorganic Analysis Laboratory – LabinAv Nossa Sra das Graças, 50. Duque de Caxias, RJ, Brazil 25250-020.

Thiago de Oliveira Araújo, Inmetro

Researcher 

 National Institute of Metrology, Quality and Technology (Inmetro)Chemical Metrology Division – Dquim/Inorganic Analysis Laboratory – LabinAv Nossa Sra das Graças, 50. Duque de Caxias, RJ, Brazil 25250-020.

Marcelo Dominguez de Almeida, Inmetro

Researcher 

 National Institute of Metrology, Quality and Technology (Inmetro)Chemical Metrology Division – Dquim/Inorganic Analysis Laboratory – LabinAv Nossa Sra das Graças, 50. Duque de Caxias, RJ, Brazil 25250-020.

María Luisa Fernández Sánchez, Universidad de Oviedo

Researcher 

Departement of Physical and Analytical Chemistry

University of Oviedo

Julián Clavería 8, 33006 Oviedo

Alfredo Sanz Medel, Universidad de Oviedo

Researcher 

Departement of Physical and Analytical Chemistry

University of Oviedo

Julián Clavería 8, 33006 Oviedo

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Published

2018-06-06